#12 Biology Class XII- Biotechnology and its Application (NEET, MHTCET) MCQ

 


1) What is antisense technology?  
(a) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene. 
(b) RNA polymerase producing DNA. 
(c) A cell displaying a foreign antigen used for synthesis of antigens. 
(d) Production of somaclonal variants in tissue cultures.
Answer 
(a) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene.

 
 
2) Cry I endotoxins obtained from Bacillus thuringiensis are effective against

(a) nematodes   
(b) bollworms 
(c) mosquitoes    
(d) flies
Answer 
(b) bollworms

 
 
3) Human insulin is being commercially produced from a transgenic species of

(a) Rhizobium    
(b) Saccharomyces 
(c) Escherichia    
(d) Mycobacterium
Answer 
(c) Escherichia   

 
 
4) Which one of the following is commonly used in transfer of foreign DNA into crop plants?

(a) Meloidogyne incognita    
(b) Agrobacterium tumefaciens 
(c) Penicillium expansum    
(d) Trichoderma harzianum
Answer 
(b) Agrobacterium tumefaciens

 
 
5) What is true about Bt toxin?

(a) Bt protein exists as active toxin in the Bacillus. 
(b) The activated toxin enters the ovaries of the pest to sterilise it and thus prevent its multiplication. 
(c) The concerned Bacillus has antitoxins. 
(d) The inactive protoxin gets converted into active form in the insect gut.
Answer 
(d) The inactive protoxin gets converted into active form in the insect gut.

 
 
6) Genetic engineering has been successfully used for producing 

(a) transgenic mice for testing safety of polio vaccine before use in humans 
(b) transgenic models for studying new treatments for certain cardiac diseases 
(c) transgenic cow-Rosie which produces high fat milk for making ghee 
(d) animals like bulls for farm work as they have super power
Answer 
(a) transgenic mice for testing safety of polio vaccine before use in humans

 
 
7) Bt cotton is not [NCERT Exemplar]

(a) a GM plant 
(b) insect resistant 
(c) a bacterial gene expressing system 
(d) resistant to all pesticides
Answer 
(d) resistant to all pesticides

 
 
8) C-peptide of human insulin is [NCERT Exemplar]

(a) a part of mature insulin molecule 
(b) responsible for formation of disulphide bridges 
(c) removed during maturation of proinsulin to insulin 
(d) tesponsible for its biological activity.
Answer 
(c) removed during maturation of proinsulin to insulin

 
 
9) GEAC stands for[NCERT Exemplar]

(a) Genome Engineering Action Committee 
(b) Ground Environment Action Committee 
(c) Genetic Engineering Approval Committee 
(d) Genetic and Environment Approval committee
Answer 
(c) Genetic Engineering Approval Committee

 
 
10) a-1-antitrypsin is [NCERT Exemplar]

(a) an antacid 
(b) an enzyme 
(c) used to treat arthritis 
(d) used to treat emphysema
Answer 
(d) used to treat emphysema

 
 
11) Human insulin is being commercially produced from a transgenic species of

(a) Rhizobium 
(b) Saccharomyces 
(c) Escherichia 
(d) Mycobacterium
Answer 
(c) Escherichia

 
 
12) A probe which is a molecule used to locate homologous sequences in a mixture of DNA or RNA molecules could be: [NCERT Exemplar]

(a) a ssRNA 
(b) a ssDNA 
(c) either RNA or DNA 
(d) can be ssDNA but not ssRNA
Answer 
(c) either RNA or DNA

 
 
13) Choose the correct option regarding retrovirus. [NCERT Exemplar]

(a) An RNA virus that synthesises DNA during infection 
(b) A DNA virus that synthesises RNA during infection 
(c) A ssDNA virus 
(d) A dsRNA virus
Answer 
(a) An RNA virus that synthesises DNA during infection

 
 
14) The site of production of ADA in the body is [NCERT Exemplar]

(a) erythrocytes 
(b) lymphocytes 
(c) blood plasma 
(d) osteocytes
Answer 
(b) lymphocytes

 
 
15) A protoxin is[NCERT Exemplar]

(a) a primitive toxin 
(b) a denatured toxin 
(c) toxin produced by protozoa 
(d) inactive toxin
Answer 
(d) inactive toxin

 
 
16) Pathophysiology is the [NCERT Exemplar]

(a) study of physiology of pathogen 
(b) study of normal physiology of host 
(c) study of altered physiology of host 
(d) none of the above
Answer 
(c) study of altered physiology of host

 
 
17) The trigger for activation of toxin of Bacillus thuringiensis is [NCERT Exemplar]

(a) acidic pH of stomach 
(b) high temperature 
(c) alkaline pH of gut 
(d) mechanical action in the insect gut
Answer 
(c) alkaline pH of gut

 
 
18) Bt corn has been made resistant from corn borer disease by introduction of the gene

(a) cryIAb 
(b) ampR 
(c) cryIIAb 
(d) trp
Answer 
(a) cryIAb

 
 
19) A bioreactor refers to

(a) fermentation tank 
(b) tank for biochemical reactions 
(c) organisms reacting to a stimulus 
(d) tank for biochemical waste
Answer 
(a) fermentation tank

 
 
20) Golden rice is [NCERT Exemplar]

(a) a variety of rice grown along the yellow river in China 
(b) long stored rice having yellow colour tint 
(c) a transgenic rice having gene for b-carotene 
(d) wild variety of rice with yellow coloured grains
Answer 
(c) a transgenic rice having gene for b-carotene

 
 
21) In RNAi, genes are silenced using [NCERT Exemplar]

(a) ssDNA 
(b) dsDNA 
(c) dsRNA 
(d) ssRNA
Answer 
(c) dsRNA

 
 
22) The first clinical gene therapy was done for the treatment of [NCERT Exemplar]

(a) AIDS 
(b) Cancer 
(c) Cystic fibrosis 
(d) SCID (Severe Combined Immuno Deficiency resulting form deficiency of ADA)
Answer 
(d) SCID (Severe Combined Immuno Deficiency resulting form deficiency of ADA)

 
 
23) ADA is an enzyme which is deficient in a genetic disorder SCID. What is the full form of ADA? [NCERT Exemplar]

(a) Adenosine deoxyaminase 
(b) Adenosine deaminase 
(c) Aspartate deaminase 
(d) Arginine deaminase
Answer 
(b) Adenosine deaminase

 
 
24) Silencing of a gene could be achieved through the use of [NCERT Exemplar]

(a) RNAi only 
(b) antisense RNA only 
(c) both RNAi and antisense RNA 
(d) none of the above
Answer 
(c) both RNAi and antisense RNA

 
 
25) Biopiracy means

(a) use of biopatents 
(b) thefts of plants and animals 
(c) stealing of bioresources 
(d) exploitation of bioresources without authentic permission
Answer 
(d) exploitation of bioresources without authentic permission

 
 
26) During the processing of ‘proinsulin’ into mature ‘insulin’

(a) A – chain is removed 
(b) C – chain is removed 
(c) B – chain is removed 
(d) No peptide chain is removed
Answer 
(b) C – chain is removed