#12 Biology Class 12 Chapter 12 - Biotechnology MHTCET / NEET MCQ
12 - Biotechnology
(1) The bacterium which causes a plant disease called crown gall is ……… .
(a) Helicobacter pylori
(b) Agrobacterium tumifaciens
(c) Thermophilus aquaticus
(d) Bacillus thuringienesis
Answer-
(b) Agrobacterium tumifaciens
(2) The enzyme nuclease hydrolyses ……… of polynucleotide chain of DNA.
(a) hydrogen bonds
(b) phosphodiester bonds
(c) glycosidic bonds
(d) peptide bonds
Answer-
(b) phosphodiester bonds
(3) In vitro amplification of DNA or RNA segment is known as ……… .
(a) chromatography
(b) southern blotting
(c) polymerase chain reaction
(d) gel electrophoresis
Answer-
(c) polymerase chain reaction
(4) Which of the following is the correct recognition sequence of restriction enzyme hind III.
(a) 5' ---A-A-G-C-T-T---3'
3' ---T-T-C-G-A-A---5'
(b) 5' ---G-A-A-T-T-C---3'
3' ---C-T-T-A-A-G---5'
(c) 5' ---C-G-A-T-T-C---3'
3' ---G-C-T-A-A-G---5'
(d) 5' ---G-G-C-C---3'
3' ---C-C-G-G---5'
Answer-
(a) 5’ ---A-A-G-C-T-T---3’
3’ ---T-T-C-G-A-A---5’
(5) Recombinant protein ......... is used to dissolve blood clots present in the body.
(a) insulin
(b) tissue plasminogen activator
(c) relaxin
(d) erythropoietin
Answer-
(b) tissue plasminogen activator
(6) Recognition sequence of restriction enzymes are generally ............... nucleotide long.
(a) 2 to 4
(b) 4 to 8
(c) 8 to 10
(d) 14 to 18
Answer-
(b) 4 to 8
(7) Recombinant DNA technique was established by ……… .
(a) Herbert Boyer
(b) Stanley Cohen
(c) Peter Lobban
(d) both (a) and (b)
Answer-
(d) both (a) and (b)
(8) Modern biotechnology includes ……… .
(a) r-DNA technology and PCR
(b) microarrays, cell culture and fusion
(c) production of curds, cheese, wine
(d) both (a) and (b)
Answer-
(d) both (a) and (b)
(9) How many of the following statements are correct with reference to electrophoresis.
1. DNA is positively charged and it moves towards the negative electrode.
2. DNA is negatively charged and it moves towards the anode.
3. Longer DNA fragments move faster during electrophoresis.
4. Shorter DNA fragments move slowly during electrophoresis.
(a) One
(b) Two
(c) Three
(d) Four
Answer-
(a) One
(10) PCR technique was developed by ……… .
(a) Stanley Cohen
(b) Herbert Boyer
(c) W. Arber
(d) K. Mullis
Answer-
(d) K. Mullis
(11) ……… technique is used for in vitro gene cloning or gene multiplication.
(a) Electrophoresis
(b) SDS-PAGE
(c) Spectroscopy
(d) PCR
Answer-
(d) PCR
(12) During denaturation step of polymerase chain reaction, the reaction mixture is heated at ……… .
(a) 40 – 60°C
(b) 70 – 75°C
(c) 90 – 98°C
(d) 36°C
Answer-
(c) 90 – 98 oC
(13) Annealing temperature of primer is ……… .
(a) 40 – 60°C
(b) 72°C
(c) 90 – 98°C
(d) 36°C
Answer-
(a) 40 – 60°C
(14) During PCR new strand of DNA is synthesized by ……… .
(a) thermostable Taq DNA polymerase
(b) ligase
(c) phosphorylase
(d) RE
Answer-
(a) thermostable Taq DNA polymerase
(15) Restriction enzymes were discovered by ……… .
(a) K. Mullis
(b) S. Cohen
(c) H. Boyer
(d) Smith, Nathan and Arber
Answer-
(d) Smith, Nathan and Arber
(16) The molecular scissors of DNA are ……… .
(a) ligases
(b) polymerases
(c) endonucleases
(d) transcriptases
Answer-
(c) endonucleases
(17) ……… generates DNA fragments with sticky ends.
(a) Eco RI
(b) Bam HI
(c) Hind II
(d) both (a) and (b)
Answer-
(d) both (a) and (b)
(18) ……… is Type III endonulcease.
(a) HapaI
(b) Eco R I
(c) BgII
(d) Eco K
Answer-
(a) HapaI
(19) Select the incorrect statement.
(a) Type II restriction endonucleases have separate activities for cleaving and methylation.
(b) Type I restriction endonucleases function simultaneously as endonuclease and methylase.
(c) Type II restriction endonucleases cut DNA at specific non–pallindromic sequences.
(d) Thousands of Type II restriction endonucleases have been discovered.
Answer-
(c) Type II restriction endonucleases cut DNA at specific non-palindromic sequences
(20) Ti plasmids are present in ……… .
(a) Bacillus thuringiensis
(b) Agrobacterium tumefaciens
(c) Haemophilus influenza
(d) Escherichia coli
Answer-
(b) Agrobacterium tumefaciens
(21) Plasmid DNA containing foreign DNA is called ……… .
(a) chimeric DNA
(b) passenger DNA
(c) recombinant DNA
(d) both (a) and (c)
Answer-
(d) both (a) and (c)
(22) Bacterial host cell takes up naked r-DNA by process of ……… .
(a) transduction
(b) transfection
(c) transformation
(d) all of these
Answer-
(c) transformation
(23) Virosomes are ……… .
(a) liposome
(b) inactivated HIV
(c) liposome + inactivated HIV
(d) none of these
Answer-
(c) liposome + inactivated HIV
(24) Recombinant protein used in the treatment of emphysema is .......... .
(a) ALPH 1-Antitrypsin
(b) relaxin
(c) Interleukin – 1 receptor
(d) urokinase
Answer-
(a) ALPH 1-Antitrypsin
(25) Tissue plasminogen activator and urokinase are used in the treatment of ……… .
(a) blood clots
(b) atherosclerosis
(c) emphysema
(d) asthama
Answer-
(a) blood clots
(26) Bacillus is involved in the production of ……… vaccine that melts in mouth.
(a) polio
(b) flu
(c) chicken pox
(d) none of these
Answer-
(b) flu
(27) First transgenic plant produced is ……… .
(a) Bt cotton
(b) flavr savr tomato
(c) wheat
(d) tobacco
Answer-
(d) tobacco
(28) Insect resistant GMO plants contain …… .
(a) cowpea trypsin inhibitor gene
(b) cry gene
(c) chalone isomerase gene
(d) (a) or (b)
Answer-
(d) (a) or (b)
(29) ‘Cry’ genes are present in ……… .
(a) Agrobacterium tumifaciens
(b) Bacillus thuringiensis
(c) Rhizobium species
(d) Escherichia coli
Answer-
(b) Bacillus thuringiensis
(30) Crystals of Bt toxin produced by some bacteria do not kill the bacteria themselves because ……… .
(a) bacteria are resistant to toxin
(b) toxin is immature
(c) toxin is inactive
(d) bacteria encloses toxin in a special case
Answer-
(c) toxin is inactive
(31) The gene coding for -amylase inhibitor isolated from ……… is transferred to tobacco.
(a) mung bean
(b) adzuki bean
(c) E.coli
(d) daffodils
Answer-
(b) adzuki bean
(32) The enzyme affecting the shelf life of flavr savr tomato is ……… .
(a) galactosidase
(b) transacetylase
(c) permease
(d) polygalactouranase
Answer-
(d) polygalactouranase
(33) Ferritin, an iron storage protein, isolated from ……… and Phaseolus is transferred to ……… to increase its iron content.
(a) soybean, rice
(b) rice, wheat
(c) maize, soybean
(d) rice, soybean
Answer-
(a) soybean, rice
(34) To improve oil content and oil quality, ……… genes are transferred to soybean, oil palm, rapeseed and sunflower.
(a) Arabidopsis
(b) cry
(c) tobacco
(d) canola
Answer-
(a) Arabidopsis
(35) Artemecin is an ……… drug developed from transgenic plants.
(a) anticancer
(b) antimalarial
(c) antibacterial
(d) antifungal
Answer-
(b) antimalarial
(36) The transgenic cow born in Scotland, could produce a human protein in her milk for human therapeutics.
(a) Dolly
(b) Molly
(c) Tracy
(d) none of these
Answer-
(c) Tracy
(37) Indian patent does not allow ……… .
(a) process patent
(b) product patent
(c) biopatent
(d) both (b) and (c)
Answer-
(b) product patent
(38) Biopatent are awarded for ……… .
(a) strains of microbes and cell lines
(b) DNA sequences
(c) GMO
(d) All of these
Answer-
(d) All of these
